Exon amplification: a strategy to isolate mammalian genes based on RNA splicing.

We have developed a method, exon amplification, for fast and efficient isolation of coding sequences from complex mammalian genomic DNA. This method is based on the selection of RNA sequences, exons, which are flanked by functional 5' and 3' splice sites. Fragments of cloned genomic DNA are inserted...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Buckler, A J, Chang, D D, Graw, S L, Brook, J D, Haber, D A, Sharp, P A, Housman, D E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51582/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1850845/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.9.4005
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!