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CryoEM structure of the spliceosome immediately after branching

Pre-mRNA splicing proceeds by two consecutive trans-esterification reactions via a lariat-intron intermediate. We present the 3.8Å cryoEM structure of the spliceosome immediately after lariat formation. The 5’-splice site is cleaved but remains close to the catalytic Mg(2+) site in the U2/U6 snRNA t...

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Publicat a:Nature
Autors principals: Galej, Wojciech P., Wilkinson, Max E., Fica, Sebastian M., Oubridge, Chris, Newman, Andrew J., Nagai, Kiyoshi
Format: Artigo
Idioma:Inglês
Publicat: 2016
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5156311/
https://ncbi.nlm.nih.gov/pubmed/27459055
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nature19316
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