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Methodology for the high-throughput identification and characterization of tRNA variants that are substrates for a tRNA decay pathway
The rapid tRNA decay (RTD) pathway is a tRNA quality control pathway known to degrade several specific hypomodified or destabilized tRNAs in the yeast Saccharomyces cerevisiae. In this chapter we describe seven methods for identifying RTD substrates, with a focus on two new approaches: a high-throug...
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| Publicado en: | Methods Enzymol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2015
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5152761/ https://ncbi.nlm.nih.gov/pubmed/26253963 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.03.003 |
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