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Methodology for the high-throughput identification and characterization of tRNA variants that are substrates for a tRNA decay pathway

The rapid tRNA decay (RTD) pathway is a tRNA quality control pathway known to degrade several specific hypomodified or destabilized tRNAs in the yeast Saccharomyces cerevisiae. In this chapter we describe seven methods for identifying RTD substrates, with a focus on two new approaches: a high-throug...

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Bibliografski detalji
Izdano u:Methods Enzymol
Glavni autori: Payea, Matthew J., Guy, Michael P., Phizicky, Eric M.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5152761/
https://ncbi.nlm.nih.gov/pubmed/26253963
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2015.03.003
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