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Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining

Gene disruption and overexpression play central roles in the analysis of gene function. Homologous recombination is, in principle, the most efficient method of disrupting, modifying, or replacing a target gene. Although homologous integration of exogenous DNA into the genome occurs readily in Saccha...

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Bibliografske podrobnosti
Main Authors: Ninomiya, Yuuko, Suzuki, Keiichiro, Ishii, Chizu, Inoue, Hirokazu
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC514464/
https://ncbi.nlm.nih.gov/pubmed/15299145
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0402780101
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