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High-resolution interrogation of functional elements in the noncoding genome

The noncoding genome affects gene regulation and disease, yet we lack tools for rapid identification and manipulation of noncoding elements. We develop a CRISPR screen employing ~18,000 sgRNAs targeting >700 kb surrounding the genes NF1, NF2, and CUL3, which are involved in BRAF inhibitor resista...

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Foilsithe in:Science
Main Authors: Sanjana, Neville E., Wright, Jason, Zheng, Kaijie, Shalem, Ophir, Fontanillas, Pierre, Joung, Julia, Cheng, Christine, Regev, Aviv, Zhang, Feng
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2016
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5144102/
https://ncbi.nlm.nih.gov/pubmed/27708104
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aaf7613
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