Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase

Homology directed repair (HDR)-based genome editing via selectable long flanking arm donors can be hampered by local transgene silencing at transcriptionally silent loci. Here, we report efficient bi-allelic modification of a silent locus in patient-derived hiPSC by using Cas9 nickase and a silencin...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Eggenschwiler, Reto, Moslem, Mohsen, Fráguas, Mariane Serra, Galla, Melanie, Papp, Oliver, Naujock, Maximilian, Fonfara, Ines, Gensch, Ingrid, Wähner, Annabell, Beh-Pajooh, Abbas, Mussolino, Claudio, Tauscher, Marcel, Steinemann, Doris, Wegner, Florian, Petri, Susanne, Schambach, Axel, Charpentier, Emmanuelle, Cathomen, Toni, Cantz, Tobias
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5133597/
https://ncbi.nlm.nih.gov/pubmed/27910942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep38198
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