Improved bi-allelic modification of a transcriptionally silent locus in patient-derived iPSC by Cas9 nickase

Homology directed repair (HDR)-based genome editing via selectable long flanking arm donors can be hampered by local transgene silencing at transcriptionally silent loci. Here, we report efficient bi-allelic modification of a silent locus in patient-derived hiPSC by using Cas9 nickase and a silencin...

Descripció completa

Guardat en:
Dades bibliogràfiques
Publicat a:Sci Rep
Autors principals: Eggenschwiler, Reto, Moslem, Mohsen, Fráguas, Mariane Serra, Galla, Melanie, Papp, Oliver, Naujock, Maximilian, Fonfara, Ines, Gensch, Ingrid, Wähner, Annabell, Beh-Pajooh, Abbas, Mussolino, Claudio, Tauscher, Marcel, Steinemann, Doris, Wegner, Florian, Petri, Susanne, Schambach, Axel, Charpentier, Emmanuelle, Cathomen, Toni, Cantz, Tobias
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 2016
Matèries:
Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5133597/
https://ncbi.nlm.nih.gov/pubmed/27910942
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep38198
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!

Ítems similars