Validation of highly sensitive simultaneous targeted and untargeted analysis of keto-steroids by Girard P derivatization and stable isotope dilution-liquid chromatography-high resolution mass spectrometry

A multiplexed quantitative method for the analysis of three major unconjugated steroids in human serum by stable isotope dilution liquid chromatography-high resolution mass spectrometry (LC-HRMS) was developed and validated on a Q. Exactive Plus hybrid quadrupole/Orbitrap mass spectrometer. This qua...

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Veröffentlicht in:Steroids
Hauptverfasser: Frey, Alexander J., Wang, Qingqing, Busch, Christine, Feldman, Daniel, Bottalico, Lisa, Mesaros, Clementina A., Blair, Ian A., Vachani, Anil, Snyder, Nathaniel W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2016
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5127713/
https://ncbi.nlm.nih.gov/pubmed/27743906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.steroids.2016.10.003
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