Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry (UPLC-HRMS)

Here we present a workflow to analyze the metabolic profiles for biological samples of interest including; cells, serum, or tissue. The sample is first separated into polar and non-polar fractions by a liquid-liquid phase extraction, and partially purified to facilitate downstream analysis. Both aqu...

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Detalhes bibliográficos
Main Authors: Snyder, Nathaniel W., Khezam, Maya, Mesaros, Clementina A., Worth, Andrew, Blair, Ian A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Assuntos:
Biochemistry
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3711276/
https://ncbi.nlm.nih.gov/pubmed/23711563
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50433
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