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Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)
Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed evolution and functional metagenomics but is currently limited to fluorescence readouts. Here we describe a highly effic...
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| Foilsithe in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
National Academy of Sciences
2016
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5127370/ https://ncbi.nlm.nih.gov/pubmed/27821774 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1606927113 |
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