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One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

[Image: see text] Directed evolution relies on iterative cycles of randomization and selection. The outcome of an artificial evolution experiment is crucially dependent on (i) the numbers of variants that can be screened and (ii) the quality of the assessment of each clone that forms the basis for s...

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Bibliographische Detailangaben
Hauptverfasser: Zinchenko, Anastasia, Devenish, Sean R. A., Kintses, Balint, Colin, Pierre-Yves, Fischlechner, Martin, Hollfelder, Florian
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Chemical Society 2014
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3952496/
https://ncbi.nlm.nih.gov/pubmed/24517505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac403585p
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