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Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

Ultrahigh-throughput screening, in which members of enzyme libraries compartmentalized in water-in-oil emulsion droplets are assayed, has emerged as a powerful format for directed evolution and functional metagenomics but is currently limited to fluorescence readouts. Here we describe a highly effic...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Gielen, Fabrice, Hours, Raphaelle, Emond, Stephane, Fischlechner, Martin, Schell, Ursula, Hollfelder, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5127370/
https://ncbi.nlm.nih.gov/pubmed/27821774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1606927113
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