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Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

Unculturable bacterial communities provide a rich source of biocatalysts, but their experimental discovery by functional metagenomics is difficult, because the odds are stacked against the experimentor. Here we demonstrate functional screening of a million-membered metagenomic library in microfluidi...

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Podrobná bibliografie
Vydáno v:Nat Commun
Hlavní autoři: Colin, Pierre-Yves, Kintses, Balint, Gielen, Fabrice, Miton, Charlotte M., Fischer, Gerhard, Mohamed, Mark F., Hyvönen, Marko, Morgavi, Diego P., Janssen, Dick B, Hollfelder, Florian
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2015
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4686663/
https://ncbi.nlm.nih.gov/pubmed/26639611
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms10008
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