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An efficient, multiply promiscuous hydrolase in the alkaline phosphatase superfamily

We report a catalytically promiscuous enzyme able to efficiently promote the hydrolysis of six different substrate classes. Originally assigned as a phosphonate monoester hydrolase (PMH) this enzyme exhibits substantial second-order rate accelerations ((k(cat)/K(M))/k(w)), ranging from 10(7) to as h...

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Detalhes bibliográficos
Main Authors: van Loo, Bert, Jonas, Stefanie, Babtie, Ann C., Benjdia, Alhosna, Berteau, Olivier, Hyvönen, Marko, Hollfelder, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2840280/
https://ncbi.nlm.nih.gov/pubmed/20133613
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0903951107
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