DNA polymerization in the absence of exonucleolytic proofreading: in vivo and in vitro studies.

Classical genetic selection was combined with site-directed mutagenesis to study bacteriophage T4 DNA polymerase 3'----5' exonuclease activity. A mutant DNA polymerase with very little (less than or equal to 1%) 3'----5' exonuclease activity was generated. In vivo, the 3'---...

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Hauptverfasser: Reha-Krantz, L J, Stocki, S, Nonay, R L, Dimayuga, E, Goodrich, L D, Konigsberg, W H, Spicer, E K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC51243/
https://ncbi.nlm.nih.gov/pubmed/2006180
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