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A CRISPR-Cas9 Assisted Non-Homologous End-Joining Strategy for One-step Engineering of Bacterial Genome

Homologous recombination-mediated genome engineering has been broadly applied in prokaryotes with high efficiency and accuracy. However, this method is limited in realizing larger-scale genome editing with numerous genes or large DNA fragments because of the relatively complicated procedure for DNA...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Su, Tianyuan, Liu, Fapeng, Gu, Pengfei, Jin, Haiying, Chang, Yizhao, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5121644/
https://ncbi.nlm.nih.gov/pubmed/27883076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep37895
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