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In vivo blunt-end cloning through CRISPR/Cas9-facilitated non-homologous end-joining
The CRISPR/Cas9 system facilitates precise DNA modifications by generating RNA-guided blunt-ended double-strand breaks. We demonstrate that guide RNA pairs generate deletions that are repaired with a high level of precision by non-homologous end-joining in mammalian cells. We present a method called...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2016
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4856974/ https://ncbi.nlm.nih.gov/pubmed/26762978 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkv1542 |
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