The intervening removable affinity tag (iRAT) production system facilitates Fv antibody fragment‐mediated crystallography

Fv antibody fragments have been used as co‐crystallization partners in structural biology, particularly in membrane protein crystallography. However, there are inherent technical issues associated with the large‐scale production of soluble, functional Fv fragments through conventional methods in var...

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Bibliographic Details
Published in:Protein Sci
Main Authors: Nomura, Yayoi, Sato, Yumi, Suno, Ryoji, Horita, Shoichiro, Iwata, So, Nomura, Norimichi
Format: Artigo
Language:Inglês
Published: John Wiley and Sons Inc. 2016
Subjects:
Methods and Applications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5119561/
https://ncbi.nlm.nih.gov/pubmed/27595817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3035
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