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The intervening removable affinity tag (iRAT) production system facilitates Fv antibody fragment‐mediated crystallography

Fv antibody fragments have been used as co‐crystallization partners in structural biology, particularly in membrane protein crystallography. However, there are inherent technical issues associated with the large‐scale production of soluble, functional Fv fragments through conventional methods in var...

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Библиографические подробности
Опубликовано в: :Protein Sci
Главные авторы: Nomura, Yayoi, Sato, Yumi, Suno, Ryoji, Horita, Shoichiro, Iwata, So, Nomura, Norimichi
Формат: Artigo
Язык:Inglês
Опубликовано: John Wiley and Sons Inc. 2016
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC5119561/
https://ncbi.nlm.nih.gov/pubmed/27595817
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3035
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