Loop electrostatics asymmetry modulates the pre-existing conformational equilibrium in thrombin

Thrombin exists as an ensemble of active (E) and inactive (E*) conformations that differ for their accessibility to the active site. Here we show that redistribution of the E*-E equilibrium can be achieved by perturbing the electrostatic properties of the enzyme. Removal of the negative charge of th...

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Bibliographic Details
Published in:Biochemistry
Main Authors: Pozzi, Nicola, Zerbetto, Mirco, Acquasaliente, Laura, Tescari, Simone, Frezzato, Diego, Polimeno, Antonino, Gohara, David W., Di Cera, Enrico, De Filippis, Vincenzo
Format: Artigo
Language:Inglês
Published: 2016
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5114853/
https://ncbi.nlm.nih.gov/pubmed/27347732
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00385
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