Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy

Stable gene transfer into target cell populations via integrating viral vectors is widely used in stem cell gene therapy (SCGT). Accurate vector copy number (VCN) estimation has become increasingly important. However, existing methods of estimation such as real-time quantitative PCR are more restric...

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Detalles Bibliográficos
Publicado en:Hum Gene Ther Methods
Autores principales: Lin, Huan-Ting, Okumura, Takashi, Yatsuda, Yukinori, Ito, Satoru, Nakauchi, Hiromitsu, Otsu, Makoto
Formato: Artigo
Lenguaje:Inglês
Publicado: Mary Ann Liebert, Inc. 2016
Materias:
Research Articles
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5111482/
https://ncbi.nlm.nih.gov/pubmed/27763786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2016.059
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