Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy

Stable gene transfer into target cell populations via integrating viral vectors is widely used in stem cell gene therapy (SCGT). Accurate vector copy number (VCN) estimation has become increasingly important. However, existing methods of estimation such as real-time quantitative PCR are more restric...

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Detalhes bibliográficos
Publicado no:Hum Gene Ther Methods
Main Authors: Lin, Huan-Ting, Okumura, Takashi, Yatsuda, Yukinori, Ito, Satoru, Nakauchi, Hiromitsu, Otsu, Makoto
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2016
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5111482/
https://ncbi.nlm.nih.gov/pubmed/27763786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2016.059
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