Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy

Stable gene transfer into target cell populations via integrating viral vectors is widely used in stem cell gene therapy (SCGT). Accurate vector copy number (VCN) estimation has become increasingly important. However, existing methods of estimation such as real-time quantitative PCR are more restric...

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Bibliographische Detailangaben
Veröffentlicht in:Hum Gene Ther Methods
Hauptverfasser: Lin, Huan-Ting, Okumura, Takashi, Yatsuda, Yukinori, Ito, Satoru, Nakauchi, Hiromitsu, Otsu, Makoto
Format: Artigo
Sprache:Inglês
Veröffentlicht: Mary Ann Liebert, Inc. 2016
Schlagworte:
Research Articles
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5111482/
https://ncbi.nlm.nih.gov/pubmed/27763786
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hgtb.2016.059
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