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Use of sequence duplication to engineer a ligand-triggered, long-distance molecular switch in T4 lysozyme

We have designed a molecular switch in a T4 lysozyme construct that controls a large-scale translation of a duplicated helix. As shown by crystal structures of the construct with the switch on and off, the conformational change is triggered by the binding of a ligand (guanidinium ion) to a site that...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Yousef, Mohammad S., Baase, Walter A., Matthews, Brian W.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 2004
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC511024/
https://ncbi.nlm.nih.gov/pubmed/15286283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404482101
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