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Resolution of organelle docking and fusion kinetics in a cell-free assay

In vitro assays of compartment mixing have been key tools in the biochemical dissection of organelle docking and fusion. Many such assays measure compartment mixing through the enzymatic modification of reporter proteins. Homotypic fusion of yeast vacuoles is measured with a coupled assay of proteol...

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Autors principals: Merz, Alexey J., Wickner, William T.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC511018/
https://ncbi.nlm.nih.gov/pubmed/15286284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404583101
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