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Resolution of organelle docking and fusion kinetics in a cell-free assay

In vitro assays of compartment mixing have been key tools in the biochemical dissection of organelle docking and fusion. Many such assays measure compartment mixing through the enzymatic modification of reporter proteins. Homotypic fusion of yeast vacuoles is measured with a coupled assay of proteol...

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Autori principali: Merz, Alexey J., Wickner, William T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC511018/
https://ncbi.nlm.nih.gov/pubmed/15286284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0404583101
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