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Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing

Membrane fusion entails organelle docking and subsequent mixing of membrane bilayers and luminal compartments. We now present an in vitro assay of fusion, using yeast vacuoles bearing domains of either Fos or Jun fused to complementary halves of β-lactamase. Upon fusion, these proteins associate to...

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Bibliografiska uppgifter
Huvudupphovsmän: Jun, Youngsoo, Wickner, William
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2007
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC1941832/
https://ncbi.nlm.nih.gov/pubmed/17664431
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0700970104
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