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Assays of vacuole fusion resolve the stages of docking, lipid mixing, and content mixing
Membrane fusion entails organelle docking and subsequent mixing of membrane bilayers and luminal compartments. We now present an in vitro assay of fusion, using yeast vacuoles bearing domains of either Fos or Jun fused to complementary halves of β-lactamase. Upon fusion, these proteins associate to...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
2007
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1941832/ https://ncbi.nlm.nih.gov/pubmed/17664431 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0700970104 |
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