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Efficient recombinant expression of SFTI-1 in bacterial cells using intein-mediated protein trans-splicing
We report for the first time the recombinant expression of bioactive wild-type sunflower trypsin inhibitor 1 (SFTI-1) inside E. coli cells by making use of intracellular protein trans-splicing in combination with a high efficient split-intein. SFTI-1 is a small backbone-cyclized polypeptide with a s...
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| Pubblicato in: | Biopolymers |
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| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2016
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5108698/ https://ncbi.nlm.nih.gov/pubmed/27178003 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.22875 |
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