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Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein

A novel strategy to modulate the assembly and trans-splicing activity of the Ssp DnaE split-intein was achieved by introducing two photolabile protecting groups onto the backbone of the C-intein polypeptide. This modification was not only able to efficiently block the trans-splicing activity, but al...

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Autors principals: Berrade, Luis, Kwon, Youngeun, Camarero, Julio
Format: Artigo
Idioma:Inglês
Publicat: 2010
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2935465/
https://ncbi.nlm.nih.gov/pubmed/20512791
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201000157
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