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Efficient and wash‐free labeling of membrane proteins using engineered Npu DnaE split‐inteins
An efficient and wash‐free method to conjugate a fluorescent tag to a target membrane protein is developed, using engineered Npu DnaE split‐inteins. This approach allowed fast labeling while avoiding the strenuous synthesis of a long polypeptide. Two different modes of labeling, namely specific bind...
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| 發表在: | Protein Sci |
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| Main Authors: | , , , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
John Wiley & Sons, Inc.
2018
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6194237/ https://ncbi.nlm.nih.gov/pubmed/30151847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3455 |
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