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In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins.

BACKGROUND: Protein trans-splicing by naturally occurring split DnaE inteins is used for protein ligation of foreign peptide fragments. In order to widen biotechnological applications of protein trans-splicing, it is highly desirable to have split inteins with shorter C-terminal fragments, which can...

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Hlavní autoři: A Sesilja Aranko, Sara Züger, Edith Buchinger, Hideo Iwaï
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science (PLoS) 2009-01-01
Edice:PLoS ONE
On-line přístup:http://europepmc.org/articles/PMC2664965?pdf=render
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