Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line

Applying clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9)-mediated mutagenesis to primary mouse immune cells, we used high-fidelity single guide RNAs (sgRNAs) designed with an sgRNA design tool (CrispRGold) to target genes in primary B cells, T ce...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Chu, Van Trung, Graf, Robin, Wirtz, Tristan, Weber, Timm, Favret, Jeremy, Li, Xun, Petsch, Kerstin, Tran, Ngoc Tung, Sieweke, Michael H., Berek, Claudia, Kühn, Ralf, Rajewsky, Klaus
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 2016
Assuntos:
Biological Sciences
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5098665/
https://ncbi.nlm.nih.gov/pubmed/27729526
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1613884113
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