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Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape

Imaging native membrane receptors and testing how they interact with ligands is of fundamental interest in the life sciences but has proven remarkably difficult to accomplish. Here, we introduce an approach that uses force-distance curve–based atomic force microscopy to simultaneously image single n...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Alsteens, David, Pfreundschuh, Moritz, Zhang, Cheng, Spoerri, Patrizia M, Coughlin, Shaun R, Kobilka, Brian K, Müller, Daniel J
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5087271/
https://ncbi.nlm.nih.gov/pubmed/26167642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3479
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