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Two New FRET Imaging Measures: Linearly Proportional to and Highly Contrasting the Fraction of Active Molecules

We developed two new FRET imaging measures for intramolecular FRET biosensors, called linearly proportional (LP) and highly contrasting (HC) measures, which can be easily calculated by the fluorescence intensities of donor and acceptor as a ratio between their weighted sums. As an alternative to the...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Yamao, Masataka, Aoki, Kazuhiro, Yukinawa, Naoto, Ishii, Shin, Matsuda, Michiyuki, Naoki, Honda
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5079603/
https://ncbi.nlm.nih.gov/pubmed/27780260
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0164254
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