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Quantitative analysis of recombination between YFP and CFP genes of FRET biosensors introduced by lentiviral or retroviral gene transfer

Biosensors based on the principle of Förster (or fluorescence) resonance energy transfer (FRET) have been developed to visualize spatio-temporal dynamics of signalling molecules in living cells. Many of them adopt a backbone of intramolecular FRET biosensor with a cyan fluorescent protein (CFP) and...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Komatsubara, Akira T., Matsuda, Michiyuki, Aoki, Kazuhiro
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4542544/
https://ncbi.nlm.nih.gov/pubmed/26290434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep13283
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