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Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy

Ratiometric quantification of CFP/YFP FRET enables live-cell time-series detection of molecular interactions, without the need for acceptor photobleaching or specialized equipment for determining fluorescence lifetime. Though popular in widefield applications, its implementation on a confocal micros...

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Main Authors: Tadross, Michael R., Park, Sarah A., Veeramani, Balaji, Yue, David T.
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2877369/
https://ncbi.nlm.nih.gov/pubmed/19196425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2008.03109.x
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