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Robust approaches to quantitative ratiometric FRET imaging of CFP/YFP fluorophores under confocal microscopy
Ratiometric quantification of CFP/YFP FRET enables live-cell time-series detection of molecular interactions, without the need for acceptor photobleaching or specialized equipment for determining fluorescence lifetime. Though popular in widefield applications, its implementation on a confocal micros...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2877369/ https://ncbi.nlm.nih.gov/pubmed/19196425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2008.03109.x |
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