Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C): Quantitative Analysis of FRET in YFP Dimers

YFP is widely used as a genetically-encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluorescence anisotropy at pH 6.0 (McAnaney, T. B., Zeng, W., Doe, C. F. E., Bhanji, N., Wakelin, S., Pearson, D. S., Abbyad, P., Shi, X...

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Main Authors: Shi, Xinghua, Basran, Jaswir, Seward, Harriet E., Childs, William, Bagshaw, Clive R., Boxer, Steven G.
格式: Artigo
語言:Inglês
出版: 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2570256/
https://ncbi.nlm.nih.gov/pubmed/18027983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701575n
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