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Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C): Quantitative Analysis of FRET in YFP Dimers

YFP is widely used as a genetically-encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluorescence anisotropy at pH 6.0 (McAnaney, T. B., Zeng, W., Doe, C. F. E., Bhanji, N., Wakelin, S., Pearson, D. S., Abbyad, P., Shi, X...

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Detalhes bibliográficos
Main Authors: Shi, Xinghua, Basran, Jaswir, Seward, Harriet E., Childs, William, Bagshaw, Clive R., Boxer, Steven G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2570256/
https://ncbi.nlm.nih.gov/pubmed/18027983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi701575n
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