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Yeast display-based antibody affinity maturation using detergent-solubilized cell lysates

It is often desired to identify or engineer antibodies that target membrane proteins (MPs). However, due to their inherent insolubility in aqueous solutions, MPs are often incompatible with in vitro antibody discovery and optimization platforms. Recently, we adapted yeast display technology to accom...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Tillotson, Benjamin J., Lajoie, Jason M., Shusta, Eric V.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5076467/
https://ncbi.nlm.nih.gov/pubmed/26060070
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2748-7_4
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