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Antibody affinity maturation using yeast display with detergent-solubilized membrane proteins as antigen sources

Antigen preparations in the form of detergent-solubilized cell lysates could, in principle, render membrane proteins (MPs) compatible with in vitro antibody engineering technologies. To this end, detergent-solubilized cell lysates were coupled with the yeast surface display platform to affinity matu...

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Detalhes bibliográficos
Main Authors: Tillotson, Benjamin J., de Larrinoa, Iñigo F., Skinner, Colin A., Klavas, Derek M., Shusta, Eric V.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3542525/
https://ncbi.nlm.nih.gov/pubmed/23109730
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzs077
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