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Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes

The S385Y/D469T/R520Q variant of E. coli transketolase was evolved previously with three successive smart libraries, each guided by different structural, bioinformatical or computational methods. Substrate-walking progressively shifted the target acceptor substrate from phosphorylated aldehydes, tow...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Affaticati, Pierre E., Dai, Shao-Bo, Payongsri, Panwajee, Hailes, Helen C., Tittmann, Kai, Dalby, Paul A.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5073344/
https://ncbi.nlm.nih.gov/pubmed/27767080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep35716
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