Directed evolution to re-adapt a co-evolved network within an enzyme

We have previously used targeted active-site saturation mutagenesis to identify a number of transketolase single mutants that improved activity towards either glycolaldehyde (GA), or the non-natural substrate propionaldehyde (PA). Here, all attempts to recombine the singles into double mutants led t...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Strafford, John, Payongsri, Panwajee, Hibbert, Edward G., Morris, Phattaraporn, Batth, Sukhjeet S., Steadman, David, Smith, Mark E.B., Ward, John M., Hailes, Helen C., Dalby, Paul A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Science Publishers 2012
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3657141/
https://ncbi.nlm.nih.gov/pubmed/22154561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jbiotec.2011.11.017
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados