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Integrated Approach to Identify Heparan Sulfate Ligand Requirements of Robo1
An integrated methodology is described to establish ligand requirements for heparan sulfate (HS) binding proteins based on a workflow in which HS octasaccharides are produced by partial enzymatic degradation of natural HS followed by size exclusion purification, affinity enrichment using an immobili...
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| Publicado no: | J Am Chem Soc |
|---|---|
| Main Authors: | , , , , , , , , , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5068570/ https://ncbi.nlm.nih.gov/pubmed/27611601 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.6b08161 |
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