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Paramagnetic Tag for Glycosylation sites in Glycoproteins: Structural Constraints on Heparan Sulfate Binding to Robo1

An enzyme- and click chemistry-mediated methodology for the site-specific nitroxide spin labeling of glycoproteins has been developed and applied. The procedure relies on the presence of single N-glycosylation sites that are present natively in proteins or that can be engineered into glycoproteins b...

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Detalhes bibliográficos
Publicado no:ACS Chem Biol
Main Authors: Moure, Maria J., Eletsky, Alexander, Gao, Qi, Morris, Laura C., Yang, Jeong-Yeh, Chapla, Digantkumar, Zhao, Yuejie, Zong, Chengli, Amster, I. Jonathan, Moremen, Kelley W., Boons, Geert-Jan, Prestegard, James H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6161356/
https://ncbi.nlm.nih.gov/pubmed/30063822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acschembio.8b00511
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