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De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides

Here, we describe the first sequencing method of a complex mixture of heparan sulfate tetrasaccharides by LC-MS/MS. Heparin and heparan sulfate (HS) are linear polysaccharides that are modified in a complex manner by N- and O-sulfation, N-acetylation, and epimerization of the uronic acid. Heparin an...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Huang, Rongrong, Zong, Chengli, Venot, Andre, Chiu, Yulun, Zhou, Dandan, Boons, Geert-Jan, Sharp, Joshua S.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5068567/
https://ncbi.nlm.nih.gov/pubmed/27087275
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.6b00519
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