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Cell-Free Systems Based on CHO Cell Lysates: Optimization Strategies, Synthesis of “Difficult-to-Express” Proteins and Future Perspectives

Nowadays, biotechnological processes play a pivotal role in target protein production. In this context, Chinese Hamster Ovary (CHO) cells are one of the most prominent cell lines for the expression of recombinant proteins and revealed as a safe host for nearly 40 years. Nevertheless, the major bottl...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Thoring, Lena, Wüstenhagen, Doreen A., Borowiak, Maria, Stech, Marlitt, Sonnabend, Andrei, Kubick, Stefan
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5042383/
https://ncbi.nlm.nih.gov/pubmed/27684475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0163670
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