Cell-free synthesis of functional human epidermal growth factor receptor: Investigation of ligand-independent dimerization in Sf21 microsomal membranes using non-canonical amino acids

Cell-free protein synthesis systems represent versatile tools for the synthesis and modification of human membrane proteins. In particular, eukaryotic cell-free systems provide a promising platform for their structural and functional characterization. Here, we present the cell-free synthesis of func...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Quast, Robert B., Ballion, Biljana, Stech, Marlitt, Sonnabend, Andrei, Varga, Balázs R., Wüstenhagen, Doreen A., Kele, Péter, Schiller, Stefan M., Kubick, Stefan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2016
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5037433/
https://ncbi.nlm.nih.gov/pubmed/27670253
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep34048
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