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A Streptavidin Binding Site Mutation Yields an Unexpected Result: An Ionized Asp128 Residue is Not Essential for Strong Biotin Binding
We report a detailed study for a point mutation of the crucial binding site residue, D128, in the biotin-streptavidin complex. The conservative substitution, D128N, preserves the detailed structure observed for the wild type complex but has only minimal impact on biotin binding, even though previous...
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| Publicado no: | Biochemistry |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5030189/ https://ncbi.nlm.nih.gov/pubmed/27603565 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00698 |
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