Second Contact Shell Mutation Diminishes Streptavidin-Biotin Binding Affinity Through Transmitted Effects on Equilibrium Dynamics

We report a point mutation in the second contact shell of the high-affinity streptavidin-biotin complex that appears to reduce binding affinity through transmitted effects on equilibrium dynamics. The Y54F streptavidin mutation causes a 75-fold loss of binding affinity with 73-fold faster dissociati...

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Detalhes bibliográficos
Main Authors: Baugh, Loren, Le Trong, Isolde, Cerutti, David S., Mehta, Nital, Gülich, Susanne, Stayton, Patrick S., Stenkamp, Ronald E., Lybrand, Terry P.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3320214/
https://ncbi.nlm.nih.gov/pubmed/22145986
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi201221j
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